N6-methyladenosine modification of hypoxia-inducible factor-1α regulates Helicobacter pylori-associated gastric cancer via the PI3K/AKT pathway

Figure 3 Knockdown of hypoxia-inducible factor-1α suppresses the PI3K/AKT pathway, epithelial-mesenchymal transition, and cell proliferation, migration, and invasion. A: Quantitative reverse transcription-polymerase chain reaction showed that hypoxia-inducible factor-1α (HIF-1α) small interfering RNA (siRNA) was successfully transfected into gastric epithelial cells (GES-1); B and C: The transfected GES-1 cells were co-cultured with H. pylori and Western blot analysis revealed the changes of HIF-1α, p-PI3K, p-AKT, epithelial-mesenchymal transition biomarkers, and invasion indicators; D: Cell counting kit-8 reagent was added into the cells with corresponding treatment and the absorbance at 450 nm was recorded; E and F: Detection of migration rate by wound healing assay. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, ^dP < 0.0001, compared with siRNA-negative control (NC) or Hp27 + siRNA-NC group. siRNA-NC: Small interfering RNA-negative control; HIF-1α: Hypoxia-inducible factor-1α.