N6-methyladenosine modification of hypoxia-inducible factor-1α regulates Helicobacter pylori-associated gastric cancer via the PI3K/AKT pathway

Figure 1 Helicobacter pylori induces hypoxia-inducible factor-1α expression and activates the PI3K/AKT pathway. A: Quantitative reverse transcription-polymerase chain reaction was performed to detect the relative mRNA level of hypoxia-inducible factor-1α (HIF-1α) at different infection time points (3 h, 6 h, 12 h, and 24 h) and multiplicity of infection (50, 100, and 200) in Helicobacter pylori-infected human gastric epithelial cells. β-actin was used as the internal reference; B and C: HIF-1α and PI3K/AKT pathway proteins were detected by Western blot analysis. β-actin was used as the internal reference. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001, compared with control group. HIF-1α: Hypoxia-inducible factor-1α.