RBM5 suppresses proliferation, metastasis and glycolysis of colorectal cancer cells via stabilizing phosphatase and tensin homolog mRNA

Figure 5 Phosphatase and tensin homolog knockdown partially reversed inhibitory effects of RNA binding motif 5 on the proliferation, metastasis and glycolysis of colorectal cancer cells. A: The knockdown efficiency of phosphatase and tensin homolog (PTEN) was detected using Western blot in HCT116 and SW480 cells; B: The expression of PTEN in HCT116 and SW480 cells was detected using Western blot; C: Cell counting kit-8 assay was used to assess the viability of HCT116 and SW480 cells; D: 5-ethynyl-2’-deoxyuridine assay was used to assess proliferation of HCT116 and SW480 cells; E and F: Migration and invasion of HCT116 and SW480 cells were evaluated by transwell assays; G: Glucose consumption, lactic acid production and adenosine-triphosphate production were detected by corresponding kits. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. PTEN: Phosphatase and tensin homolog.