RBM5 suppresses proliferation, metastasis and glycolysis of colorectal cancer cells via stabilizing phosphatase and tensin homolog mRNA

Figure 3 RNA binding motif 5 binds to phosphatase and tensin homolog mRNA to stabilize its expression. A: Relative protein expression of phosphatase and tensin homolog (PTEN) in HCT116 and SW480 cells was detected by Western blot after RNA binding motif 5 (RBM5) overexpression; B: Relative protein expression of PTEN and RBM5 in HCT116 and SW480 cells was detected by Western blot; C: The correlation between the expression of RBM5 and PTEN in colon adenocarcinoma and rectum adenocarcinoma; D: The binding of RBM5 and PTEN in HCT116 and SW480 cells was confirmed by RNA immunoprecipitation assay; E: Relative mRNA expression of PTEN in HCT116 and SW480 cells was detected by quantitative real time-polymerase chain reaction after treatment with 5 μg/mL actinomycin D for 0-2-4-6-8 hours; F: CatRAPID (http://service.tartaglialab.com/page/catrapid_group) was used to predict the binding regions between RBM5 and PTEN 3 ‘UTR; G: Dual-luciferase reporter assay was used to confirm the interaction between RBM5 and PTEN 3’UTR. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. RBM5: RNA binding motif 5; PTEN: Phosphatase and tensin homolog.