RBM5 suppresses proliferation, metastasis and glycolysis of colorectal cancer cells via stabilizing phosphatase and tensin homolog mRNA

Figure 2 RNA binding motif 5 overexpression represses proliferation, migration, invasion and glycolysis of colorectal cancer cells. A: Relative protein expression of RNA binding motif 5 in HCT116 and SW480 cells was detected by Western blot; B: Cell counting kit-8 (CCK-8) assay was used to assess the viability of HCT116 and SW480 cells; C: 5-ethynyl-2’-deoxyuridine assay was used to assess proliferation of HCT116 and SW480 cells; D and E: Migration and invasion of HCT116 and SW480 cells were evaluated by transwell assays; F: Glucose consumption, lactic acid production, and adenosine-triphosphate production were detected by corresponding kits; G: Protein levels of hexokinase 2 and lactate dehydrogenase A were detected by Western blot in HCT116 and SW480 cells. RBM5: RNA binding motif 5; EDU: 5-ethynyl-2’-deoxyuridine; HK2: Hexokinase 2; LDHA: Lactate dehydrogenase A. ^bP < 0.01, ^cP < 0.001.