Aldehyde dehydrogenase 2 family member repression promotes colorectal cancer progression by JNK/p38 MAPK pathways-mediated apoptosis and DNA damage

Figure 5 Aldehyde dehydrogenase 2 family member inhibited MAPK-apoptosis and DNA damage by regulating acetaldehyde in colorectal cancer cells. A: Phosphorylation of JNK and P38 MAPK in CL-40 and SK-CO-1 cells in the presence of acetaldehyde (ACE) (200 μM) were measured by western blot; B: Flow cytometric analysis of apoptosis of CL-40 and SK-CO-1 cells in the presence of ACE; C: Detection of protein level of γH2AX in CL-40 and SK-CO-1 cells with the presence of ACE via western blot; D: The cell viability in CL-40 and SK-CO-1 cells with the presence of ACE was measured by cell counting kit-8. Data are displayed as the mean ± SD. ^cP < 0.001.