Aldehyde dehydrogenase 2 family member repression promotes colorectal cancer progression by JNK/p38 MAPK pathways-mediated apoptosis and DNA damage

Figure 2 Aldehyde dehydrogenase 2 family member promotes the accumulated acetaldehyde and DNA damage of colorectal cancer cells. A: Western blot measured the transfection efficiency of shRNA aldehyde dehydrogenase 2 family member (sh-ALDH2) and treated with Alda-1 (1 μM) in CL-40 and SK-CO-1 cells; B: Quantitative reverse transcriptase PCR measured the transfection efficiency of sh-ALDH2 and treated with Alda-1 (1 μM) in CL-40 and SK-CO-1 cells; C: Acetaldehyde quantification of sh-ALDH2 and sh-ALDH2+Alda-1 cells; D: Western blot measured the γH2AX (a DNA-damage response protein) expression of CL-40 and SK-CO-1 cells; E: Comet assay of sh-ALDH2 transfected CL-40 and SK-CO-1 cells that were treated with or without Alda-1. Data are displayed as the mean ± SD. ^bP < 0.01, ^cP < 0.001. ALDH2: Aldehyde dehydrogenase 2 family member.