Weiwei Decoction alleviates gastric intestinal metaplasia through the olfactomedin 4/nucleotide-binding oligomerization domain 1/caudal-type homeobox gene 2 signaling pathway

Figure 4 Weiwei Decoction reduces the expression of intestinal markers KLF transcription factor 4, villin 1, and MUCIN 2 in intestinal metaplasia rats. A: Western blot detection of villin 1 (VIL1) and KLF transcription factor 4 (KLF4) in rat gastric mucosa; B: Relative protein expression of VIL1 and KLF4 (n = 4); C: Relative mRNA expression of MUCIN 2 (MUC2) and VIL1 in gastric mucosa (n = 5); D: Immunohistochemistry detection of MUC2, representative images of the gastric mucosa in each group. Scale bar: 100 μm; E: Quantitative analysis of MUC2 protein expression in gastric tissues (n = 3). ^aP < 0.01, ^bP < 0.001 vs control group; ^cP < 0.05, ^dP < 0.01, ^eP < 0.001 vs model group; ^fP < 0.05, ^gP < 0.01, ^hP < 0.001 vs folic acid group. VIL1: Villin 1; KLF4: KLF transcription factor 4; WWD: Weiwei Decoction; FA: Folic acid; MUC2: MUCIN 2.