Yiqi Jiedu Huayu decoction inhibits precancerous lesions of chronic atrophic gastritis by inhibiting NLRP3 inflammasome-mediated pyroptosis

Figure 4 Yiqi Jiedu Huayu decoction inhibited NLRP3 inflammasome activation by inactivating TLR4/NF-κB and IL-6/STAT3 pathways. A: We performed an orally taken of N-methyl-N’-nitro-N-nitrosoguanidine and Helicobacter pylori in rats to induce chronic atrophic gastritis with precancerous lesion combined with Yiqi Jiedu Huayu decoction treatment, and TLR4, p-p65, p65, p-STAT3 and STAT3 protein levels in gastric mucosa were assessed by western blot (n = 10); B-D: The interaction between NLRP3 promoter between H3K9ac/p-STAT3/p-65 was analyzed using chromatin immunoprecipitation assay. The measurement data were presented as mean ± SD. All data were obtained from at least three replicate experiments. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. YJHD: Yiqi Jiedu Huayu decoction; IgG: immunoglobulin G.