Kombo knife combined with sorafenib in liver cancer treatment: Efficacy and safety under immune function influence

Figure 6 Detection of immune function in the peripheral blood of hepatocellular carcinoma patients before and after treatment. A: Flow cytometry was used to isolate CD3+ T cells from the peripheral blood mononuclear cells (PBMCs) of healthy donors, patients before and after treatment with Kombo knife, and patients before and after combined treatment with Kombo knife and sorafenib for hepatocellular carcinoma (HCC); B: Flow cytometry was used to isolate CD4+ T cells and CD8+ T cells from the peripheral blood of healthy donors, patients before and after treatment with Kombo knife, and patients before and after combined treatment with Kombo knife and sorafenib for HCC treatment; C: Flow cytometry was used to isolate natural killer cells from the PBMCs of healthy donors, patients before and after treatment with Kombo knife, and patients before and after combined treatment with Kombo knife and sorafenib for HCC treatment; D: Flow cytometry was used to isolate Treg cells from the PBMCs of healthy donors, patients before and after treatment with Kombo knife, and patients before and after combined treatment with Kombo knife and sorafenib for HCC treatment; E: The numbers of CD3+ T cells, CD4+ T cells, and CD8+ T cells were counted before and after treatment in each treatment group, and the ratios of CD4+ T cells to CD8+ T cells were compared; F: Enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of Th1 and Th2 cytokines in peripheral blood; G: ELISA was used to measure the level of heat shock protein 70 in peripheral blood; H and I: The expression of peptidylprolyl isomerase A was detected using real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR) (H) and Western blot (I) techniques; J and K: The expression of solute carrier family 29 member 3 was detected using RT-qPCR (J) and Western blot (K) techniques. All the groups included 32 patients, with mean ± SD values. ^aP < 0.05, indicates a significant difference between groups with P < 0.05. NK: Natural killer cell; IL: Interleukin.