Integrated single-cell and bulk RNA sequencing revealed an epigenetic signature predicts prognosis and tumor microenvironment colorectal cancer heterogeneity

Figure 8 Cell-cell interaction and the signature. A: Although most interactions exist in both low and high-risk samples; B: The interaction number is significantly different; C and D: Despite that the interaction number is higher in high-risk samples, the interaction strength is lower; E: The macrophage is noticed as the most significantly altered immune cell among the interactions; F: The pathway level interaction information flow was visualized for both groups, overall; G: Top 10 for each group; H: Among these interactions, it is noticed that macrophage is especially active in high-risk samples while interactions involved CD8+ T cell were increased in the low-risk group; I: Specific pathways and cell type interaction landscape also revealed that macrophage is activated in high-risk samples.