N-glycosylation of Wnt3 regulates the progression of hepatocellular carcinoma by affecting Wnt/β-catenin signal pathway

Figure 4 Loss of N-glycosylation from Wnt3 weakens cell proliferation, invasion, migration and colony formation. A and B: Wound healing experiments were performed 36 h after PLC/PRF/5 cell transfection; C and D: At 36 h after transfection, the MHCC97H cells were subjected to wound healing experiments; E and F: At 36 h following the transfection of PLC/PRF/5 cells, after the cell count, 500 cells were removed for colony formation experiments; G and H: At 36 h after MHCC97H cell transfection, after the cell count, 500 cells were removed for colony formation experiments; I: Real-time cellular analysis (RTCA) was used to detect the proliferation of PLC/PRF/5 cells every 20 minutes for a total of 80 h; J: RTCA was used to detect the invasive ability of PLC/PRF/5 cells every 15 min for a total of 60 h; K: RTCA was used to detect the proliferation of MHCC97H cells every 20 min for a total of 80 h. ^aP < 0.01.