N-glycosylation of Wnt3 regulates the progression of hepatocellular carcinoma by affecting Wnt/β-catenin signal pathway

Figure 3 Site-directed mutations in Wnt3 affect signaling pathways downstream. A: The effect of different Wnt3 N-glycosylation-deficient mutants on the expression of effector molecules in the Wnt/β-catenin signaling pathway was assessed by Western blot analysis; B: ImageJ software was used to detect the IntDen values, and the p-β-catenin/GAPDH values were compared between the groups; C: ImageJ software was used to detect the IntDen values, and the Cyclin D1/GAPDH values were compared between the groups; D: ImageJ software was used to detect the IntDen values, and the C-myc/GAPDH values were compared between the groups; E: ImageJ software was used to detect the IntDen values, and the CDC42/GAPDH values were compared between the groups; F: ImageJ software was used to detect the IntDen values, and the Survivin/GAPDH values were compared between the groups. The data are presented as the mean ± SD from three independent experiments. ^aP < 0.05, ^bP < 0.01. GFP: Green fluorescent protein.