Circ_0004592: An auxiliary diagnostic biomarker for gastric cancer

Figure 3 Features of circ_0004592. A: Schematic illustration of the circ_0004592 formation from STK3 gene in the chromosome 8; B: The existence of circ_0004592 was confirmed by real-time fluorescent quantitative polymerase chain reaction (RT-PCR) and AGE using convergent and divergent primers. Circ_0004592 can only be amplified in cDNA. GAPDH serves as control; C: Stability of circ_0004592 and linear STK3 was assessed by RNase treatment followed by quantitative RT-PCR (qRT-PCR); D: Stability of circ_0004592 and linear STK3 was assessed by Actinomycin D treatment followed by qRT-PCR at different time points.