Aspirin suppresses hepatocellular carcinoma progression by inhibiting platelet activity

Figure 2 Platelets promote hepatocellular carcinoma cell invasion and epithelial-mesenchymal transition. A and B: Transwell assays were used to detect the effects of platelet plasma (PPP) and platelet-rich plasma (PRP) on hepatocellular carcinoma (HCC) cell invasion, and the statistical results are shown on the right. 200 × magnification, Scale bar: 200 μm; C and D: Transwell assays were used to detect the effects of platelets (PLTs) on HCC cell invasion, and the statistical results are shown on the right. 200 × magnification, Scale bar: 200 μm; E and F: Real time polymerase chain reaction (RT-PCR) was used to detect the effects of PPP and PRP on the epithelial-mesenchymal transition (EMT) ability of HCC cells; G and H: RT-PCR was used to detect the effect of PLTs on the EMT ability of HCC cells. The figure shows the mean ± SD of the experimental results, with asterisks denoting significant differences between the two groups (^aP < 0.05, ^bP < 0.01, ^cP < 0.001). PPP: Platelet plasma; PRP: Platelet-rich plasma; FBS: Fetal bovine serum; PLT: Platelets.