Shi-pi-xiao-ji formula suppresses hepatocellular carcinoma by reducing cellular stiffness through upregulation of acetyl-coA acetyltransferase 1

Figure 7 Western blot analysis of acetyl-coA acetyltransferase 1 protein expression levels. A: Representative image shows the expression of acetyl-coA acetyltransferase 1 (ACAT1) protein in vehicle, Shi-pi-xiao-ji (SPXJ)-serum, methyl-β-cyclodextrin (MβCD), and water-soluble cholesterol (Chol) groups of MHCC97H and HepG2 cells. Actin was used as loading control; B: Histogram plots show the relative expression levels of ACAT1 protein in the vehicle, SPXJ-serum, MβCD, and Chol groups of MHCC97H and HepG2 cells. The results are expressed as means ± SEM (n= 3). ^aP < 0.01, ns: P > 0.05. SPXJ: Shi-pi-xiao-ji; MβCD: Methyl-β-cyclodextrin; Chol: Water-soluble cholesterol; ACAT1: Acetyl-coA acetyltransferase 1.