Shi-pi-xiao-ji formula suppresses hepatocellular carcinoma by reducing cellular stiffness through upregulation of acetyl-coA acetyltransferase 1

Figure 4 Flow cytometry results show the percentage of apoptotic hepatocellular carcinoma cells in the vehicle, Shi-pi-xiao-ji-serum, methyl-β-cyclodextrin, and water-soluble cholesterol groups of MHCC97H cells and HepG2 cells based on the annexin V/PI double-staining. A and B: Flow cytometry results show the percentage of apoptotic hepatocellular carcinoma (HCC) cells in the vehicle, Shi-pi-xiao-ji (SPXJ)-serum, methyl-β-cyclodextrin (MβCD), and water-soluble cholesterol (Chol) groups of MHCC97H cells based on the annexin V/PI double-staining; C and D: Flow cytometry results show the percentage of apoptotic HCC cells in the vehicle, SPXJ-serum, MβCD, and Chol groups of HepG2 cells based on the annexin V/PI double-staining. The results are expressed as means ± SEM (n= 3). ^aP < 0.01, ^bP < 0.001 and ns: P > 0.05. SPXJ: Shi-pi-xiao-ji; MβCD: Methyl-β-cyclodextrin; Chol: Water-soluble cholesterol; PI: Propidium iodide.