Bidirectional effects of the tryptophan metabolite indole-3-acetaldehyde on colorectal cancer

Figure 4 Indole-3-acetaldehyde promotes invasiveness and epithelial-mesenchymal transition of colorectal cancer cell lines. A: Crystal violet staining image of cells in the lower chamber (100 ×); B: Quantitative analysis of cell numbers in the lower chamber; C: Changes in the mRNA expression levels of cadherin 1, cadherin 2, occludin, snail family transcriptional repressor 1, and vimentin in the HCT116 and DLD-1 cells; D: Western blotting was used to detect the expression of intracellular cadherin; E: The expression and subcellular localization of cadherin were determined by immunofluorescence. ^aP < 0.05 vs control group; ^bP < 0.01 vs control group. DAPI: 4’,6-diamidino-2-phenylindole; CDH1: Cadherin 1; CDH2: Cadherin 2; OCLN: Occludin; SNAI1: Snail family transcriptional repressor 1; VIM: Vimentin.