Bidirectional effects of the tryptophan metabolite indole-3-acetaldehyde on colorectal cancer

Figure 2 Indole-3-acetaldehyde inhibits proliferation of colorectal cancer cells in vitro. A and B: Growth curves of HCT116 and DLD-1 cells treated with IAAD; C and D: Vitality of HCT116 and DLD-1 cells treated with different concentrations of indole-3-acetaldehyde after 48 h, compared with the control group; E and F: The expression of Ki-67 in the nucleus was determined by immunofluorescence. ^aP < 0.05, 50 μmol/L indole-3-acetaldehyde treatment group vs control group; ^bP < 0.01, 50 μmol/L indole-3-acetaldehyde treatment group vs control group; ^dP < 0.01, 100 μmol/L indole-3-acetaldehyde treatment group vs control group. DAPI: 4’,6-diamidino-2-phenylindole.