Complement factor I knockdown inhibits colon cancer development by affecting Wnt/β-catenin/c-Myc signaling pathway and glycolysis

Figure 8 Complement factor I silencing inhibited glycolysis in colon cancer cells by regulating the Wnt/β-catenin/c-Myc signaling pathway. A and B: Protein expression levels of β-catenin, c-Myc, glucose transporter 1, hexokinase 2, lactate dehydrogenase A, and pyruvate kinase M2 were examined by western blotting in HT29 cells in the sh-NC, sh-CFI, and sh-CFI + lithium chloride groups; C: In HT29 cells, glucose absorption and lactate generation are detected. ^bP < 0.01, ^cP < 0.001 vs sh-NC group; ^dP < 0.05, ^eP < 0.01 vs sh-CFI group. CFI: Complement factor I; GLUT1: Glucose transporter 1; HK2: Hexokinase 2; LDHA: Lactate dehydrogenase A; PKM2: Pyruvate kinase M2; LiCl: Lithium chloride.