Complement factor I knockdown inhibits colon cancer development by affecting Wnt/β-catenin/c-Myc signaling pathway and glycolysis

Figure 7 Complement factor I knockdown suppressed Wnt/β-catenin/c-Myc signaling pathway and glycolysis in colon cancer model mice. A: Western blotting was used to detect the protein expression levels of β-catenin and c-Myc in tumor tissues; B: Protein expression levels of key enzymes of the glycolytic pathway (glucose transporter 1, hexokinase 2, lactate dehydrogenase A and pyruvate kinase M2) were measured by western blot; C: Glucose uptake and lactate production in tumor tissue. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs Lv-sh-NC group (n = 6 mice per group). CFI: Complement factor I; GLUT1: Glucose transporter 1; HK2: Hexokinase 2; LDHA: Lactate dehydrogenase A; PKM2: Pyruvate kinase M2.