Complement factor I knockdown inhibits colon cancer development by affecting Wnt/β-catenin/c-Myc signaling pathway and glycolysis

Figure 5 Complement factor I knockdown inhibits Wnt/β-catenin/c-Myc signaling pathway and glycolytic metabolism in colon cancer cells. A and B: Detection of β-catenin and c-Myc protein expression levels by western blotting after knockdown of complement factor I (CFI) in HT29 cells and HCT116 cells; C-F: The sh-CFI vector was transfected into HT29 and HCT116 colon cancer (CC) cells, and western blotting was used to examine the protein expression levels of key enzymes in the glycolytic pathway, including glucose transporter 1, hexokinase 2, lactate dehydrogenase A and pyruvate kinase M2 (C and E); glucose uptake and lactate production in HT29 and HCT116 CC cells (D and F). ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs sh-NC group. CFI: Complement factor I; GLUT1: Glucose transporter 1; HK2: Hexokinase 2; LDHA: Lactate dehydrogenase A; PKM2: Pyruvate kinase M2.