Complement factor I knockdown inhibits colon cancer development by affecting Wnt/β-catenin/c-Myc signaling pathway and glycolysis

Figure 4 Knockdown of complement factor I inhibited proliferation, migration and invasion of colon cancer cells. A: Quantitative reverse transcription polymerase chain reaction analysis was conducted to evaluate the effectiveness of complement factor I (CFI) silencing; B: CCK-8 assay was performed to assess the viability of colon cancer cells in each group; C: Cell proliferation capability was measured by 5-ethynyl-2’-deoxyuridine assay; D: At 0 h and 24 h, the area inside the scratched area was measured, and the wound healing assay was used to evaluate cell migration; E: Using the transwell assay, the cellular invasion was assessed. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs sh-NC group. CFI: Complement factor I; EdU: 5-ethynyl-2’-deoxyuridine.