Association between Helicobacter pylori infection, mismatch repair, HER2 and tumor-infiltrating lymphocytes in gastric cancer

Table 4 Multivariate analysis of factors associated with disease-free survival and overall survival

Features	DFS		OS
	HR	P value	HR	P value
Age
< 60	Reference	-	Reference	-
> 60	1.8 (1.2, 2.5)	0.002	1.9 (1.3, 2.8)	< 0.001
Histological grade
Differentiated	Reference		Reference
Undifferentiated	1.7 (1.01, 2.7)	0.043	2.0 (1.2, 3.3)	0.007
WHO classification
1-2	Reference		Reference
3-4	2.5 (1.6, 3.8)	< 0.001	2.4 (1.5, 3.7)	< 0.001
Clinical stage
I-II	Reference		Reference
III	2.1 (1.4, 3-2)	< 0.001	1.9 (1.2, 2.9)	0.004
IT CD3/HPF
High (> 58)	Reference		Reference
Low (< 58)	1.5 (1.02, 2.2)	0.037	1.6 (1.1, 2.4)	0.018
IT CD8/CD3 ratio
< 0.63	Reference		Reference
> 0.63	1.7 (1.2, 2.5)	0.002	1.6 (1.1, 2.3)	0.008
Non-significant variables
Sex	-	0.939	-	0.51
Cardia-fundus	-	0.398	-	0.212
Bormann	-	0.328	-	0.315
Lauren	-	0.311	-	0.291
Lymph nodes	-	0.836	-	0.663
lymphovascular invasion	-	0.972	-	0.277
H. pylori	-	0.123	-	0.140
Deficient mismatch-repair	-	0.973	-	0.365
HER2	-	0.222	-	0.114
IT TIL	-	0.940	-	0.547
Stromal TIL	-	0.818	-	0.806
IT CD8/HPF	-	0.077	-	0.340

DFS: Disease-free survival; OS: Overall survival; HR: Hazard ratio; WHO: World Health Organization; IT: Intratumoral; TIL: tumor-infiltrating lymphocytes; HPF: High power field; H. pylori: Helicobacter pylori.