Association between Helicobacter pylori infection, mismatch repair, HER2 and tumor-infiltrating lymphocytes in gastric cancer

Table 3 Relationship between density of infiltrating immune cells and clinico-pathological features

Features	IT CD3	P value	ST CD3	P value	IT CD8	P value	ST CD8	P value	IT CD163	P value
Median	> 58		> 95		> 70		> 68		> 240
Age		0.714		0.275		0.026		0.224		0.162
< 60	73		47		42.1		32.9		7.4
≥ 60	71.5		52.8		52.7		39.3		13.5
Sex		0.039		0.557		0.814		0.699		0.354
Female	76.5		52		48.4		37.7		8.7
Male	67.8		49		47.3		35.7		12.7
Gastric region location		0.503		0.585		0.795		0.901		0.031
No antrum	74.1		52.4		48.7		36.2		16.7
Antrum	71.1		49.4		47.4		36.9		7.1
Bormann		0.648		0.441		0.338		0.006		0.462
I-II	70.4		47.4		41.8		22.6		5.3
III	73.6		55.6		50.7		43		11.7
IV-V	68.9		52.9		46.1		30		13
Lauren histology		0.03		0.003		0.308		0.003		0.038
Intestinal	66.2		57.4		43.7		40.3		8.2
Diffuse	78		36.6		50		22.2		17.8
Mixed	75.9		50.8		52.7		46.3		2.9
Grade		<0.001		0.311		<0.001		0.083		0.02
1	59		55.3		26.8		20.5		3.8
2	62.9		54.2		40.7		38.2		4.3
3	79.5		46.4		55.3		39.2		16.4
Lymphovascular invasion		0.602		0.241		0.092		0.117		0.865
No	73.9		55.2		41.8		30.4		11.3
Yes	71.5		48.4		50.5		39.3		10.5
Lymph node involvement		0.723		0.983		0.487		0.141		0.252
Yes	72.6		50.4		48.9		39.1		12.1
No	71		50.5		45.2		30.6		6.3
Pathology stage		0.942		0.101		0.674		0.058		0.197
I	70.9		50		42.9		20		9.5
II	73.2		59		47.2		41		4.1
III	72		46.3		49.2		37.8		13.3
Recurrence		0.02		0.079		0.448		0.475		0.389
No	76		53.9		46.6		35.2		9.3
Yes	65.9		44.4		50		39.1		13.2
H. pylori HE		0.547		0.817		0.356		0.97		0.637
Absent	71.1		51.4		49.1		36		13.4
Present	73.9		52.8		44.2		36.2		11.1
H. pylori qPCR		0.143		0.335		0.001		0.001		0.105
Absent	83.1		55.9		62.7		49.1		26.1
Present	74		48.1		37		22.8		14.1
CagA/H. pylori+		0.254		0.603		0.023		0.54		0.729
CagA-	82.9		42.9		57.5		21.2		14.3
CagA+	74.2		48		37.2		26.6		17.2
VacAs/H. pylori+		0.992		0.843		0.071		0.855		0.823
VacAs-	76.8		45.8		51.7		24.4		15.2
VacAs+	76.9		47.6		37.3		25.9		16.9
VacAs1/H. pylori+		0.397		0.432		0.942		0.234		0.543
VacAs1-	79.2		53.5		36.4		18.4		14.7
VacAs1+	72.2		44.7		37		30		20.8
VacAm/H. pylori+		0.1		0.248		0.255		0.884		0.865
VacAm-	69.4		40.4		47.6		24.5		17.1
VacAm+	80.6		50.6		38.7		25.6		15.8
VacAm1/H. pylori+		0.145		0.098		0.242		0.822		0.877
VacAm1-	89.7		65.2		48.1		23.8		16.7
VacAm1+	77		44.8		35.4		26.3		15.2
VacAs1+m1+/H. pylori+		0.113		0.289		0.913		0.253		0.345
No VacAs1+m1+	86		57.5		37.7		21.6		12.9
VacAs1+m1+	72.2		44		38.9		34.6		23.5
dMMR		0.393		0.049		0.095		0.05		0.169
No	75.4		47.6		45.5		32.3		11.5
Yes	71.3		59.5		54.7		43.9		5.1
HER2 status		0.06		0.204		0.009		0.404		0.835
Negative	74.4		52.4		49.9		37.7		11.1
Positive	56.5		38.1		21.7		28.6		9.1

HE: Hematoxylin and eosin; IT: Intratumoral; ST: Stromal; HPF: High power field; H. pylori: Helicobacter pylori; dMMR: Deficient mismatch-repair; qPCR: Quantitative PCR.