Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells via the PI3K/AKT pathway

Figure 3 Heterogeneous ribonucleoprotein A1-b enhances the Warburg effect in SW620 and HCT116 cells. A: Quantitative real-time polymerase chain reaction analysis was performed in SW620 and HCT116 cells transfected with heterogeneous ribonucleoprotein A1 (hnRNPA1)-b overexpression or hnRNPA1-b silenced to detect PKM1 and PKM2 expression; B: SW620 and HCT116 cells were transfected with hnRNPA1-b overexpressed plasmid or hnRNPA1-b interfering sequence and the expression of PKM1 and PKM2 was detected by western blot. GAPDH was used as the internal reference; C and D: SW620 and HCT116 cells were transfected with control or hnRNPA1 overexpression plasmid or silenced hnRNPA1-b for 48 h. Then glucose uptake and lactate production were measured. Scale bar: 100 μm. ^bP < 0.01, ^cP < 0.001 vs OE-NC group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001 vs si-NC group, n = 3. OE-NC: Overexpression normal control; OE-A1-b: Overexpression of hnRNPA1-b; si-NC: Knock-down normal control; si-RNA2: Knockdown of hnRNPA1-b group 2; si-RNA3: Knockdown of hnRNPA1-b group 3.