Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells via the PI3K/AKT pathway

Figure 2 Heterogeneous ribonucleoprotein A1-b promotes colon cancer cell proliferation. A: The effect of heterogeneous ribonucleoprotein A1 (hnRNPA1)-b overexpression or silencing on hnRNPA1-b expression was analyzed by quantitative real-time polymerase chain reaction analysis; B: SW620 and HCT116 cells expressing hnRNPA1-b were evaluated using cell counting kit-8 assays to assess cell viability; C: 5-ethynyl-2’-deoxyuridine incorporation assays were performed in hnRNPA1-b overexpressed or hnRNPA1-b silenced SW620 and HCT116 cells. The percentage of proliferating cells (red fluorescence) to total cells (blue fluorescence) was calculated. Scale bar: 100 μm; D and E: Flow cytometry was performed to evaluate the effect of hnRNPA1-b overexpressed or hnRNPA1-b silenced on the cell cycle distribution and apoptosis. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs OE-NC group; ^dP < 0.05, ^eP < 0.01, ^fP < 0.001, ns: P > 0.05 vs si-NC group, n = 3. EdU: 5-ethynyl-2’-deoxyuridine; OE-NC: Overexpression normal control; OE-A1-b: Overexpression of hnRNPA1-b; si-NC: Knock-down normal control; si-RNA2: Knockdown of hnRNPA1-b group 2; si-RNA3: Knockdown of hnRNPA1-b group 3.