Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells via the PI3K/AKT pathway

Figure 1 Heterogeneous ribonucleoprotein A1-b expression is upregulated in colon cancer and correlated with colon cancer progression. A: The mRNA expression level of heterogeneous ribonucleoprotein A1 (hnRNPA1)-b in colon cancer (CC) cell lines (SW480, HCT116, HT29, and SW620) and normal colon epithelial cell NCM460 were detected by quantitative real-time polymerase chain reaction; B: The mRNA expression level of hnRNPA1-b is upregulated in CC tissues relative to paired paracancerous and normal tissues; C: The relationship between hnRNPA1-b expression level and clinical stage of CC was analyzed by immunohistochemistry. Scale bar: 100 μm. The arrow indicates positive cells expressing hnRNPA1-b. ^aP < 0.05, ^bP < 0.001 vs NCM460 group; ^cP < 0.05 vs adjacent group; ^dP < 0.05 vs stage I group; ^eP < 0.05 vs stage III group; ^fP > 0.05 vs normal group; ^gP > 0.05 vs stage II group, n = 3. hnRNPA1: Heterogeneous ribonucleoprotein A1; MOD: Mean optical density.