Identification of differentially expressed mRNAs as novel predictive biomarkers for gastric cancer diagnosis and prognosis

Figure 11  Glutamic-pyruvic transaminase upregulation suppresses GC cell proliferation, migration and invasion. A: Real-time quantitative polymerase chain reaction (RT-qPCR) of glutamic-pyruvic transaminase (GPT) mRNA level in gastric cancer cell lines (AGS, MKN45, MKN28 and HGC27) and normal immortalized cell line (GES1); B and C: Western blotting of GPT protein level; D-F: Overexpression efficiency of GPT overexpression vector was verified by RT-qPCR and western blotting; G and H: CCK-8 assays of cell viability; I and J: Flow cytometry of cell cycle; K and L: Transwell assays of cell migration and invasion. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001.