SERPINH1 promoted the proliferation and metastasis of colorectal cancer by activating PI3K/Akt/mTOR signaling pathway

Figure 3 Knockdown of serpin peptidase inhibitor clade H member 1 significantly inhibited proliferation, migration and invasion in colorectal cancer. A: Knockdown of serpin peptidase inhibitor clade H member 1 (SERPINH1) was confirmed at the protein and mRNA level in HCT8 cells by western blotting and quantitative real-time polymerase chain reaction. mean ± SD (n = 3); B-L: Cell counting kit 8, colony and 5-ethynyl-2’-deoxyuridine assays suggest that knockdown of SERPINH1 inhibited the proliferation of colorectal cancer (CRC) cells (B, C, D, E, I). Knockdown of SERPINH1 inhibited the migration and invasion of CRC cells (F, G). Knockdown of SERPINH1 promoted the apoptosis of CRC cells (H, J); K and L: Knockdown of SERPINH1 inhibited the G0/G1 ratio of CRC cells. All the data are presented as the mean ± SEM. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. SERPINH1: Serpin peptidase inhibitor clade H member 1.