SERPINH1 promoted the proliferation and metastasis of colorectal cancer by activating PI3K/Akt/mTOR signaling pathway

Figure 2 Overexpression of serpin peptidase inhibitor clade H member 1 significantly promoted proliferation, migration and invasion in colorectal cancer. A: Overexpression of serpin peptidase inhibitor clade H member 1 (SERPINH1) was confirmed at the protein and mRNA level in HCT116 cells by western blotting and quantitative real-time polymerase chain reaction. mean ± SD (n = 3); B-L: Cell counting kit 8, colony and 5-ethynyl-2’-deoxyuridine assays suggest that overexpression of SERPINH1 promoted the proliferation of colorectal cancer (CRC) cells (B, C, D, E, I). Overexpression of SERPINH1 promoted the migration and invasion of CRC cells (F, G). Overexpression of SERPINH1 inhibited the apoptosis of CRC cells (H, J); K and L: Overexpression of SERPINH1 promoted the G0/G1 ratio of CRC cells. All the data are presented as the mean ± SEM. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. SERPINH1: Serpin peptidase inhibitor clade H member 1.