MicroRNA-298 determines the radio-resistance of colorectal cancer cells by directly targeting human dual-specificity tyrosine(Y)-regulated kinase 1A

Figure 5 MicroRNA-298 knockdown regulates radio-induced apoptosis depended on human dual-specificity tyrosine(Y)-regulated kinase 1A expression. A: Western blotting was carried out to examine human dual-specificity tyrosine(Y)-regulated kinase 1A (DYRK1A) levels in HT-29 and HT-29-IR cell lines; B: HT-29 cells were exposed to radiation following empty vector or pcDNA-DYRK1A vector transfection. Clonogenic assay was performed to assess cell survival; C: HT-29 cells were treated with radiation exposure after empty vector or pcDNA-DYRK1A vector transfection, followed by cell apoptosis examination by Annexin V/PI staining; D: HT-29 cells were treated with radiation exposure after empty vector or pcDNA-DYRK1A vector transfection, and cell viability was then determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; E: HT-29 cells were transfected with inhibitor, or co-transfected with inhibitor and si-DYRK1A, and then exposed to radiation. Western blotting was carried out to measure cleaved-caspase 3/9 protein levels. ^aP < 0.05.