MicroRNA-298 determines the radio-resistance of colorectal cancer cells by directly targeting human dual-specificity tyrosine(Y)-regulated kinase 1A

Figure 3 MicroRNA-298 overexpression decreases p53 binding protein 1 expression. A: After scramble or microRNA-298 (miR-298) mimic transfection, HT-29 cells were detected by Western blotting for ATM, H2AX, and p53 binding protein 1 (53BP1) protein levels; B: HT-29 cells were subjected to radiation exposure after scamble or miR-298 mimic transfection. Western blotting was performed to detect the total and phosphorylated ATM, H2AX, and 53BP1 expression. ^aP < 0.05, ^bP < 0.01. miR-298: MicroRNA-298; IR: Ionizing radiation; ATM: Ataxia telangiectasia mutated; H2AX: H2A.X variant histone; 53BP1: p53 binding protein 1.