MicroRNA-298 determines the radio-resistance of colorectal cancer cells by directly targeting human dual-specificity tyrosine(Y)-regulated kinase 1A

Figure 1 MicroRNA-298 overexpression promoted the radiation resistance of colorectal cancer cell strains. A: As demonstrated by clonogenic assay, radiation-resistant cell lines (HT-29-IR) exhibited a notable increase in survival rates compared to the radio-sensitive parental cell lines (HT-29) upon exposure to radiation treatment; B: Annexin V/PI staining was performed on HT-29 and HT-29-IR exposed to 5 Gy irradiation. The results indicated that HT-29-IR cells were highly resistant to radiation-induced apoptotic cell death than HT-29; C: Real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) was performed to determine microRNA-298 (miR-298) expression in HT-29 and HT-29-IR; D: RT-qPCR was conducted to assess miR-298 levels in HT-29 transfection with either the miR-298 mimic or miR-298 inhibitor; E: HT-29 cells were transfected with miR-298 mimic or miR-298 inhibitor and then exposed to radiation, followed by cell survival assessment by clonogenic assay. ^aP < 0.05, ^bP < 0.01. miR-298: MicroRNA-298; HT-29-IR: Radiation-resistant cell lines; IR: Ionizing radiation.