NOX4 promotes tumor progression through the MAPK-MEK1/2-ERK1/2 axis in colorectal cancer

Figure 4 Nicotinamide adenine dinucleotide phosphate oxidase 4 induces colorectal cancer progression. A: Venn diagram showing the overlap of metabolic differentially expressed genes and genes correlated with poor prognosis; B: Quantitative real-time polymerase chain reaction analysis of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4) expression in tumor and adjacent normal tissues; C: Immunohistochemical representative images of NOX4 expression in colorectal cancer patient samples; D: Quantification of NOX4 expression in tumor and adjacent normal tissues in tumor samples; E: Kaplan-Meier curves showing the overall survival between high and low-NOX4 expression groups in our dataset; F and G: Clone and sphere formation was evaluated in NOX4-overexpressing RKO (RKO-OE) and wild-type (RKO-Ctrl) cells; H-J: Cell invasion and migration of RKO-OE and RKO-Ctrl cells were measured using the transwell chamber assay and scratch assay. ^aP < 0.05, ^bP < 0.01. DEG: Differentially expressed gene; NOX4: Nicotinamide adenine dinucleotide phosphate oxidase 4.