Establishment of a cholangiocarcinoma risk evaluation model based on mucin expression levels

Figure 4 Detoxification function of MUC5B-high tumor cells of cholangiocarcinoma. A: Top 50 enriched signaling pathways of the differentially expressed genes in MUC5B-high cells; B: Heatmap showing the relative activities of oxidation-related metabolic signaling pathways in MUC5B-high vs MUC5B-low cells; C: Violin plots showing relative expression levels of GSTA1, GSTA2, ALDH1A1, ALDH2, UCP2, and MUC5B in cholangiocarcinoma (CCA) tumor sub-clusters; D: Gene set enrichment analysis plot showing the enrichment of genes from MUC5B-high (left) and MUC5B-low (right) cells in the drug resistance signaling pathway. The P value and normalized enrichment score are indicated on the plot; E: Distribution of MUC5B and GSTA1 in the spatial transcriptomics (ST) slide. Zoomed in portions of the ST chip (middle) showing multiple areas of co-localization; F: Correlation analysis between the expression of MUC5B and ALDH1A1 (left), MUC5B and UCP2 (right) in CCA tumor samples from cohort 10.