Success rate of current human-derived gastric cancer organoids establishment and influencing factors: A systematic review and meta-analysis

Table 4 The quality assessment according to our adjusted Methodological Index for Non-Randomized Studies tool

Criteria	Tong et al[37], 2023	Steele et al[24], 2019	Nanki et al[23], 2018	Yan et al[21], 2018	Li et al[25], 2022	Kawasaki et al[22], 2020	Li et al[27], 2023	Zou et al[26], 2022
A clearly stated aim	2	2	2	2	2	2	2	2
Inclusion of consecutive patients	2	2	2	2	2	2	2	2
Prospective collection of data	2	2	2	2	2	2	2	2
Endpoints appropriate to the aim of the study	2	2	2	2	2	2	2	2
Follow-up period appropriate to the aim of the study	2	1	2	2	1	2	2	1
Loss to follow up less than 5%	2	1	2	2	2	2	2	1
Baseline equivalence of groups	2	2	2	2	1	1	1	2
Adequate statistical analyses	2	2	2	2	2	2	2	2
Total	16	15	16	16	14	15	15	14

The items are scored 0 (not reported), 1 (reported but inadequate) or 2 (reported and adequate). Criteria: (1) A clearly stated aim: The question addressed should be precise and relevant in the light of available literature; (2) Inclusion of consecutive patients: All patients potentially fit for diagnostic criteria of gastric cancer have been included in the study during the study period; (3) Prospective collection of data: Data were collected according to a protocol established before the beginning of the study; (4) Endpoints appropriate to the aim of the study: Unambiguous explanation of the criteria of successfully culture; (5) Follow-up period appropriate to the aim of the study: The follow-up should be sufficiently long at least for 30 d to allow the assessment of the stable construction; (6) Loss to follow up less than 5%: All gastric cancer organoids should be included in the follow up. Otherwise, the proportion fail to follow up should not exceed the proportion experiencing the major endpoint; (7) Baseline equivalence of groups: The groups should be similar regarding the criteria other than the studied endpoints. Absence of confounding factors that could bias the interpretation of the results; and (8) Adequate statistical analyses: The statistics were in accordance with the type of study.