CALD1 facilitates epithelial-mesenchymal transition progression in gastric cancer cells by modulating the PI3K-Akt pathway

Figure 2 Inhibition of CALD1 in gastric cancer cell lines reduces cell activity, migration, and invasion, and alters epithelial-mesenchymal transition marker expression. A: CALD1 expression levels were higher in gastric cancer cell lines HGC27, NCI-N87, AGS, and MKN45 than in gastric epithelial cell line GES-1, with the strongest expression found in AGS and MKN45 cells; B: The inhibitory effect of CALD1-siRNA was verified; C: After transfection of AGS and MKN45 cells with CALD1-siRNA, cell activity was significantly reduced, and the migration and invasion ability was decreased; D: The expression of E-cadherin and Claudin-1 was increased in AGS and MKN45 cells after inhibiting the expression of CALD1, while the expression of N-cadherin, Vimentin, and Dickkop-1 mRNA and protein was decreased. ^aP < 0.05, ^bP < 0.01.