Ubiquitin-specific protease 21 promotes tumorigenicity and stemness of colorectal cancer by deubiquitinating and stabilizing ZEB1

Figure 3 Ubiquitin-specific protease 21 strengthened cell proliferation, migration and stemness. Groups were separated into the sh-NC, sh-USP21 and sh-USP21+pcDNA3.1/ZEB1 group. A: The cell proliferation was detected through colony formation assay; B and C: The cell migration and invasion abilities were determined through Transwell assay; D: The stemness was tested through sphere formation assay. ^cP < 0.001. NC: Negative control; USP21: Ubiquitin-specific protease 21.