Ubiquitin-specific protease 21 promotes tumorigenicity and stemness of colorectal cancer by deubiquitinating and stabilizing ZEB1

Figure 2 Ubiquitin-specific protease 21 remained the stability of ZEB1. A: The interaction between USP21 and ZEB1 was evaluated through Co-IP assay; B: The interaction between USP21 and ZEB1 was detected through GST pull down assay; C: The mRNA and protein expressions of USP21 were examined after USP21 knockdown through real time-quantitative polymerase chain reaction (RT-qPCR) and western blot; D: The mRNA expression of ZEB1 was tested after suppressing USP21 through RT-qPCR; E: The protein expression of ZEB1 was examined after silencing USP21 through western blot; F: The protein expression of ZEB1 was assessed after cyclohexane treatment through western blot; G: The protein expression of ZEB1 was measured after MG132 treatment through western blot; H: The ubiquitination level of ZEB1 was evaluated through western blot. ^bP < 0.01, ^cP < 0.001. NC: Negative control; CHX: Cyclohexane; USP21: Ubiquitin-specific protease 21.