Comprehensive analysis of the protein phosphatase 2A regulatory subunit B56ε in pan-cancer and its role and mechanism in hepatocellular carcinoma

Figure 7 Effects of B56ε downregulation on the proliferation, invasion, and migration of hepatocellular carcinoma cells. A: Quantitative polymerase chain reaction (qPCR) detected the expression of B56ε in human hepatocellular carcinoma (HCC) cell lines; B: qPCR and western blotting verified B56ε knockdown efficiency; C: Downregulation of B56ε expression on the proliferation of HCC cells was determined by the Cell Counting Kit-8 assay; D: Downregulation of B56ε expression on the proliferation of HCC cells was determined by the colony formation assay; E and F: Wound healing assay (E) and transwell assay (F) showed the ability of cells to invade and migrate after downregulation of B56ε. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. NC: Negative vector control; KD-1: B56ε knockdown vector 1; KD-2: B56ε knockdown vector 2; CCK-8: Cell Counting Kit-8.