Prohibitin 1 inhibits cell proliferation and induces apoptosis via the p53-mediated mitochondrial pathway in vitro

Figure 6 Prohibitin 1 is involved in intrinsic mitochondrial signalling pathway activation. A-K: HepG2 and SMMC-7721 cells were transfected with pEGFP-PHB1 or shRNA-PHB1 for 48 h. Cytochrome C protein levels in the cytosol (C) and mitochondrium (M) were determined via Western blot analyses (A-C); p53, Bax, Bcl-2, caspase 3, and caspase 9 mRNA and protein levels were determined by real-time PCR (D-G) and Western blot (H-K), respectively. The data are presented as the mean ± SD of three independent experiments with similar results. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001 vs control and empty vector/shRNA-control. Cyt C: Cytochrome C.