Matrine promotes colorectal cancer apoptosis by downregulating shank-associated RH domain interactor expression

Figure 4 Matrine induces apoptosis and regulates apoptotic proteins by downregulating shank-associated RH domain interactor. A: The apoptosis rate of colorectal cancer (CRC) cells in 4 groups [control group (con group), SHARPIN knockdown group (siSHARPIN group), matrine, siSHARPIN + matrine]; B: The expression of caspase 3, caspase 8, B-cell lymphoma-2 (Bcl-2) associated X protein, Bcl-2 associated agonist of cell death, Bcl-2 proteins in CRC cells in 4 groups; C: The suppressing effect of matrine on shank-associated RH domain interactor (SHARPIN) in CRC cells. ^aP < 0.05; ^bP < 0.01; ^cP < 0.05 vs con,^dP < 0.01 vs con; ^eP < 0.05 vs siSHARPIN,^fP < 0.01 vs siSHARPIN; ^gP < 0.05 vs matrine,^hP < 0.01 vs matrine; Con: Control group; siSHARPIN: SHARPIN knockdown group; matrine: Matrine group; siSHARPIN + matrine: Matrine and SHARPIN knockdown group; CRC: Colorectal cancer; SHARPIN: Shank-associated RH domain interactor; Bcl-2: B-cell lymphoma-2; BAD: Bcl-2 associated agonist of cell death; BAX: Bcl-2 associated X protein.