Vitamin D 1,25-Dihydroxyvitamin D3 reduces lipid accumulation in hepatocytes by inhibiting M1 macrophage polarization

doi:10.4251/wjgo.v16.i12.4685

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Dec 15, 2024 (publication date) through Nov 23, 2024

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World Journal of Gastrointestinal Oncology

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World J Gastrointest Oncol. Dec 15, 2024; 16(12): 4685-4699
Published online Dec 15, 2024. doi: 10.4251/wjgo.v16.i12.4685

Table 1 Murine primers

Primer	Forward (5′-3′)	Reverse (5′-3′)
iNOS2	GTGTTCCACCAGGAGATGTTG	CTCCTGCCCACTGAGTTCGTC
TNFα	TCTTCTCATTCCTGCTTGTGG	GGTCTGGGCCATAGAACTGA
IL-6	GTTCTCTGGGAAATCGTGGA	GGAAATTGGGGTAGGAAGGA
Arg1	CTCCAAGCCAAAGTCCTTAGAG	AGGAGCTGTCATTAGGGACATC
Mrc2	TACAGCTCCACGCTATGGATT	CACTCTCCCAGTTGAGGTACT
IL-10	GTTACTTGGGTTGCCAAG	TTGATCATCATGTATGCTTC
VDR	GCTTCCACTTCAACGCTAT	ACTCCTTCATCATGCCAAT
PPARγ	GCCCTTTACCACAGTTGATTTCT	GTGATTTGTCCGTTGTCTTTCCT
IL-1β	CCCAAGCAATACCCAAAGAA	TTGTGAGGTGCTGATGTACCA
SREBP1C	ACAGCAACCAGAAGCTCAAG	TGCCCTCCATAGACACATCT
FASN	TTGGGTGCTGACTACAACCT	TGGATGATGTTGATGATGGA
ACOX1	ACCAGCCCAACTGTGACTTC	ACAAAGGCATGTAACCCGTA
CPT1A	CTTCCCATTTGACACCTTTG	ATACGTGAGGCAGAACTTGC
GAPDH	CCTTCCGTGTTCCTACCC	CAACCTGGTCCTCAGTGTAG

iNOS2: Nitric oxide synthase; TNFα: Tumor necrosis factor α; IL: Interleukin; Arg1 Arginine 1; Mrc2: Macrophage mannose receptor 2; VDR: Vitamin D receptor; PPARγ: Peroxisome proliferator activated receptor γ; SREBP1C: Sterol-regulatory element binding protein 1C; FASN: Fatty acid synthase; CPT1A: Carnitine palmitoyl transferase 1A.

Citation: Luo WJ, Dong XW, Ye H, Zhao QS, Zhang QB, Guo WY, Liu HW, Xu F. Vitamin D 1,25-Dihydroxyvitamin D₃ reduces lipid accumulation in hepatocytes by inhibiting M1 macrophage polarization. World J Gastrointest Oncol 2024; 16(12): 4685-4699
URL: https://www.wjgnet.com/1948-5204/full/v16/i12/4685.htm
DOI: https://dx.doi.org/10.4251/wjgo.v16.i12.4685