Jianpi-Huatan-Huoxue-Anshen formula ameliorates gastrointestinal inflammation and microecological imbalance in chemotherapy-treated mice transplanted with H22 hepatocellular carcinoma

Figure 6 Tzu-Chi cancer-antagonizing & life-protecting II decoction regulates mRNA expression of colonic ZO-1, Occludin, Claudin-1, MUC-2, NF-κB, and IκB-α in H22 tumor mice receiving chemotherapy. A-F: The mRNA expression analysis of ZO-1 (A), Occludin (B), Claudin-1 (C), MUC-2 (D), NF-κB (E), and IκB-α (F). G: Gel electrophoretograms of the amplification products of ZO-1, NF-κB, Occludin, Claudin-1, IκB-α, MUC-2, and β-actin obtained after real-time PCR. Data with error bars are represented as the mean ± SD (n = 6). ^aP < 0.05 vs NS-Contr; ^bP < 0.01 vs NS-Contr; ^cP < 0.05 vs 5-fluorouracil (FU)-Contr; ^dP < 0.01 vs 5-FU-Contr; ^eP < 0.05 vs Yangzhengxiaoji capsules, one-way ANOVA. 5-FU: 5-fluorouracil; YZXJ: Yangzhengxiaoji capsules; TCCL-H: High-dose Tzu-Chi cancer-antagonizing & life-protecting II decoction; TCCL-M: Medium-dose Tzu-Chi cancer-antagonizing & life-protecting II decoction; TCCL-L: Low-dose Tzu-Chi cancer-antagonizing & life-protecting II decoction.