Jianpi-Huatan-Huoxue-Anshen formula ameliorates gastrointestinal inflammation and microecological imbalance in chemotherapy-treated mice transplanted with H22 hepatocellular carcinoma

Figure 5 Tzu-Chi cancer-antagonizing & life-protecting II decoction regulates protein expression levels of colonic ZO-1, MUC-2, NF-κB, Occludin, Claudin-1, and IκB-α in H22 hepatocellular carcinoma mice after chemotherapy. A-F: Immunohistochemical analysis of ZO-1 (A), Occludin (B), Claudin-1 (C), MUC-2 (D), NF-κB (E), and IκB-α (F) expression; G-R1: Immunohistochemical images of ZO-1 (G-L), Occludin (M-R), Claudin-1 (S-X), MUC-2 (A1-F1), NF-κB (G1-L1), and IκB-α (M1-R1) in different groups. G, M, S, A1, G1, and M1: NS-Contr (saline-treated control); H, N, T, B1, H1, and N1: 5-fluorouracil (FU)-Contr (25 mg/kg); I, O, U, C1, I1, and O1: Yangzhengxiaoji (YZXJ) capsules (0.78 g/kg); J, P, V, D1, J1, and P1: Tzu-Chi cancer-antagonizing & life-protecting II decoction (TCCL)-H (17.6 g/kg); K, Q, W, E1, K1 and Q1: TCCL-M (8.8 g/kg); L, R, X, F1, L1 and R1: TCCL-L (4.4 g/kg). The scale bars for G to R1 are 100 μm. Data with error bars are represented as the mean ± SD (n = 6 or 7). ^aP< 0.05 vs NS-Contr; ^bP < 0.01 vs NS-Contr; ^cP < 0.05 vs 5-FU-Contr; ^dP < 0.01 vs 5-FU-Contr; ^eP < 0.05 vs YZXJ capsules, one-way ANOVA. 5-FU: 5-fluorouracil; YZXJ: Yangzhengxiaoji capsules; TCCL-H: High-dose Tzu-Chi cancer-antagonizing & life-protecting II decoction; TCCL-M: Medium-dose Tzu-Chi cancer-antagonizing & life-protecting II decoction; TCCL-L: Low-dose Tzu-Chi cancer-antagonizing & life-protecting II decoction.