Long noncoding RNA steroid receptor RNA activator 1 inhibits proliferation and glycolysis of esophageal squamous cell carcinoma

Figure 4 MiRNA-363-5p directly targets phospholysine phosphohistidine inorganic pyrophosphate phosphatase in esophageal squamous cell carcinoma cells. A: Quantitative reverse transcription-polymerase chain reaction analysis indicated that miRNA-363-5p expression remarkably decreased after si-miRNA-363-5p transfection; B: Inhibition of miRNA-363-5p expression significantly attenuated proliferation in EC9706 and TE1 cells; C: Inhibition of miRNA-363-5p expression significantly attenuated glycolysis in EC9706 and TE1 cells; D: Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP) was predicted to have binding sequences for miRNA-363-5p; E: Luciferase reporter turned out that miRNA-363-5p mimic clearly weakened the luciferase activity of WT-LHPP; F: Downregulating miRNA-363-5p enhanced the LHPP protein levels in EC9706 and TE1 cells; G: LHPP expression was low in esophageal squamous cell carcinoma tissues compared to adjacent normal tissues; H: MiRNA-363-5p is negatively correlated with LHPP. ^aP < 0.05 vs NC; ^bP < 0.01 vs NC; ^cP < 0.01 vs Adjacent. NC: Negative control; LHPP: Phospholysine phosphohistidine inorganic pyrophosphate phosphatase.