MicroRNA-363-3p inhibits colorectal cancer progression by targeting interferon-induced transmembrane protein 1

Figure 2 Interferon-induced transmembrane protein 1 is a direct target of microRNA-363-3p. A: The complementary sequences of microRNA-363-3p were identified in 3’ untranslated region of interferon-induced transmembrane protein 1 (IFITM1) mRNA; B: Quantitative polymerase chain reaction (qPCR) analysis of IFITM1 mRNA expression in SW1116 cells transfected with agomir-363-3p or agomir-NC; C: Western blot analysis of IFITM1 expression in SW1116 cells transfected with agomir-363-3p or agomir-NC. Graph shows quantification of relative levels of IFITM1 expression; D: qPCR analysis of IFITM1 mRNA expression in SW480 cells transfected with antagomir-363-3p or antagomir-NC; E: Western blot analysis of IFITM1 expression in SW480 cells transfected with antagomir-363-3p or antagomir-NC. Graph shows quantification of relative levels of IFITM1 expression; F and G: Luciferase reporter gene assays using constructs containing the predicted targeting sequence (pGL3-wt) and mutated targeting sequence (pGL3-mt). SW1116 cells were transfected with the indicated constructs. The results were shown as the mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. UTR: Untranslated region; IFITM1: Interferon-induced transmembrane protein 1; miR-363-3p: MicroRNA-363-3p; NC: Negative control; NS: Not significant.