Osteopontin promotes gastric cancer progression via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway

Figure 3 Osteopontin upregulates matrix metalloproteinase 2 and vascular endothelial growth factor expression. A: Matrix metalloproteinase 2 mRNA level was tested by real-time quantitative-reverse transcription polymerase chain reaction (PCR) and normalized to β-Actin expression; B: Vascular endothelial growth factor mRNA level was detected by quantitative PCR and normalized to β-Actin expression. Data are shown as the means ± SE (n = 3). ^bP < 0.01. MMP-2: Matrix metalloproteinase 2; VEGF: Vascular endothelial growth factor; NC-shRNA: Negative control shRNA.