Osteopontin promotes gastric cancer progression via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin signaling pathway

Figure 1 Expression levels of osteopontin and capacity of proliferation in human gastric cancer cell lines. A: real-time quantitative-reverse transcription analysis of osteopontin (OPN) mRNA levels in the gastric cancer (GC) cell lines (AGS, SGC-7901, HGC-27) and normal human gastric mucosal epithelial cell line (GES-1); B: Western blot assay of OPN protein expression levels in various human GC cell lines; C: Densitometry analysis of the protein bands of OPN proteins; D: The relative proliferation rate at 0, 24, 48 and 96 h in various GC cells by 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di- phenytetrazoliumromide assay. Data are shown as the means ± SE (n = 3). ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. OPN: Osteopontin.